Composite

Part:BBa_K1368000:Design

Designed by: Zhixiang Liu   Group: iGEM14_HZAU-China   (2014-09-30)


pc1+lox71+TetR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we built this part, we often failed because of indel in the part.The tetR protein may be poison. so if you want to build other parts like this, you can try to add tetracycline in the LB.

Source

All the basic parts come from iGEM distribution.

References